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. 2018 Jan 18;8:1075. doi: 10.1038/s41598-017-18996-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Areca nut-induced calcium responses are limited to the aqueous fraction and engender both calcium release and calcium entry. Average [Ca²⁺]_i responses before and following the application of 50 µg/ml Areca nut’s total, aqueous or organic (chloroform) extract in the presence (2 Ca) or absence (0 Ca) of external 2 mM CaCl₂. The measurements were performed using RBL-2H3 rat mast cells (A), Jurkat human T cells (B), U937 human monocytes (C), HL-60 human neutrophils (D) and human primary Peripheral Blood Mononuclear Cells (PBMCs) from two different sources; PBMCs-1 from Lonza (E) and PBMCs-2 from Stemcell Technologies (F).