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. 2018 Jan 18;8:1108. doi: 10.1038/s41598-017-18697-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of the A3B4 subunit in transgenic soybean seeds. SDS-PAGE and western blotting of total proteins in the control and transgenic soybean seeds accumulating the A3B4 subunit. (A) Total proteins were extracted from soybean seeds using SDS buffer. Proteins (12 μg) were loaded onto a 12% SDS-PAGE gel. 1, the normal cultivar (cv. Jack); 2, the JQ line; 3, transgenic soybean. (B) Soybean seed extracts were analysed by western blotting using an anti-A3B4 antibody. 1, the normal cultivar (cv. Jack) accumulating glycinin and β-conglynin; 2, the JQ line; 3, transgenic soybean. Arrow indicates the band corresponding to an acidic chain of A3B4 subunit. Anti-A3B4 antibody was cross-reacted with acidic chains of glycinin group I (A1a, A1b, and A2) as a second band in lane 1.