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. 2018 Jan 18;8:1063. doi: 10.1038/s41598-018-19599-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Suppression of v-Src expression in NIH3T3 cells. (a–f) NIH3T3/TR/v-Src-wt cells were treated with 1 µg/ml Dox for the indicated times. (a–c) Cells were fixed and doubly stained with anti-Src (#327) and anti-pTyr antibodies for flow cytometry. (d) Cells were fixed and stained with anti-p21 antibody for flow cytometry. (e) Cells were fixed and doubly stained with anti-Src (#327) and anti-p21 antibodies for flow cytometry. Two-dimensional histograms (Src vs p21) are presented. (f) Cells were fixed and doubly stained with anti-Src (#327) and anti-pERK1/2 antibodies for flow cytometry. Src and pERK histograms (left) and two-dimensional histograms (Src vs pERK) (right) are presented. (g) NIH3T3/TR/v-Src(K295M) cells were treated with 1 µg/ml Dox for the indicated times. Cells were fixed and stained with anti-Src (#327) antibody for flow cytometry.