Figure 5.
Re-adhesion of cells and formation of piled-up transformed foci by v-Src expression. (a,b) NIH3T3/TR/v-Src-wt cells (1 × 105 cells /60-mm dish) were cultured for 1 day and treated (a) without or (b) with 1 µg/ml Dox on day 0, and the same field images were continuously observed over 11 days of culture by phase-contrast microscopy. The cells forming piled-up transformed foci are marked by dotted lines. Scale bars, 100 µm. (c) Colony focus formation assay. NIH3T3/TR/v-Src-wt cells at the indicated cell numbers were seeded in 60-mm dishes, cultured for 1 day, and treated with 1 µg/ml Dox for 12 days. The number of piled-up transformed foci was counted. Results represent means ± S.D. from three independent experiments.