Figure 7.

Chromosome abnormalities upon v-Src expression. (a–c) NIH3T3/TR/v-Src-wt cells were treated with 1 µg/ml Dox for the indicated times. (a) Cells were doubly stained with anti-Src (#327) antibody and PI. Magnified images of the squared areas are shown on the right. An arrow shows v-Src-negative non-adherent cell. Scale bars, 10 µm. (b) Cells were doubly stained with anti-cleaved caspase-3 antibody and PI. Arrows show non-apoptotic chromosome aberrant cells. Scale bars, 10 µm. (c) NIH3T3/TR/v-Src-wt cells were treated with 1 µg/ml Dox for the indicated times. Cells were fixed and doubly stained with anti-Src (#327) antibody and PI for flow cytometry. Two-dimensional histograms (Src vs DNA) (left) are presented. Red horizontal lines separate the region of v-Src expression (upper region) from the region of v-Src suppression (lower region). DNA histograms of the region of v-Src-expression (middle) and the region of v-Src suppression (right) are shown. The peaks of G₁ and G₂/M phases in v-Src-uninduced cells are marked by red vertical dotted lines. The two histograms at 0 h of Dox addition are the same control data (v-Src-uninduced cells), which enable us to easily compare differences in DNA content per cell during v-Src induction.