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. 2018 Jan 18;8:1138. doi: 10.1038/s41598-018-19489-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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KLF5 expression levels are positively correlated with MIT sensitivity in breast cell lines (A). MIT decreased cell viability of breast cell lines. Eleven breast cell lines were plated in 48-well plates at a density of 1 × 10⁴ − 4 × 10⁴/well. One day after cell seeding, the cells were treated with MIT or DMSO control at indicated dosages for another 48 hours. The cells were then fixed for the SRB assay to measure cell viability. (B) The protein expression of KLF5, ERα, PR and HER2 in 11 breast cell lines was measured by immunoblotting. β-actin was used as a loading control.