Fig. 2.

KLF2 and KLF4 transcriptionally regulate LEENE. a Putative KLF2 and KLF4 binding sites in LEENE enhancer/promoter based on the conserved KLF2 and KLF4 binding motifs (shown on the top). Middle tracks display H3K27ac and H3K4me1 ChIP-seq signals in LEENE locus, and the inset shows H3K4me3 ChIP-seq signals in the putative LEENE promoter region from ENCODE HUVEC data; arrows indicate regions detected in ChIP-qPCR (Fig. 2d). b–d HUVECs were infected with respective adenoviruses for 48 h. RNA levels of KLF2, KLF4, LEENE, and eNOS were detected by qPCR (in b and c) and KLF4 binding to promoters of eNOS and LEENE was quantified by ChIP-qPCR analysis (in d). e qPCR of respective RNA levels in ECs transfected with scramble control (Ctrl) or KLF2 siRNA. Data are presented as mean ± SEM, n = 5 in each group. *p < 0.05 compared to respective controls using Student’s t test