Fig. 7.

LEENE homolog in mouse. a Comparison of human LEENE and mouse BY707159.1 loci. b Putative KLF2/4 TFBS in DNA region encoding BY707159.1, indicated by blue (for KLF2) and red (for KLF4). c Sequence alignment between human LEENE and BY707159.1. d Measurement of BY707159.1 RNA levels in thoracic aorta (TA) and aortic arch (AA) using qPCR. e qPCR of BY707159.1 and eNOS RNA level in isolated mouse lung ECs transfected with scramble or LEENE LNA. In each experiment, lungs from four animals were pooled for isolation and transfection. Data are average from four independent experiments. Error bars present mean ± SEM. *p < 0.05 between AA and TA in d and between scramble and LNA in e based on Student’s t test