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. 2018 Jan 18;8:1147. doi: 10.1038/s41598-018-19413-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of TFP on the apoptosis of SWOZ2, SWOZ2-BCNU and U251 cells. (A) Flow cytometry analysis of Annexin V-positive cells in SWOZ2, SWOZ2-BCNU and U251 cells incubated with TFP or control. (B–D) The rate of Annexin V-positive cells in SWOZ2, SWOZ2-BCNU and U251 cells after treatment. All Annexin V-positive cells were considered apoptotic cells, and their percentage was calculated among the total number of cells. (E) TUNEL staining assay on SWOZ2, SWOZ2-BCNU and U251 cells after TFP treatment (400×). (F–H) The rate of apoptotic cells in SWOZ2, SWOZ2-BCNU and U251 cells after treatment by TUNEL assay. Each bar represents the mean ± SD of three experiments. *P < 0.05 compared with the control.