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. 2018 Jan 18;8:1103. doi: 10.1038/s41598-018-19429-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Catalytic activity of recombinant PDIs when performing isomerization of opMPD to clMPD. (A) Three time curves of the disulfide switch by using a 1:1, a 5:1, or a 10:1 ratio of opMPD to PDIA6. The percentage of clMPD was taken as measure of activity. (B) SDS-PAGE of four different samples was used to confirm the integrity of PDIA6 after incubation. (C) Purity of recombinant PDIs was verified by SDS PAGE. (D) RP-HPLC chromatogram of the conversion of opMPD to clMPD by reduced PDIs. Green line: non-treated opMPD; Black line: MPD treated with PDIA1; Dotted black line: MPD treated with PDIA3; Red line: MPD treated with PDIA6. (E) Histogram of the average PDI activities (relative to PDIA6) as calculated by peak areas from the data given in (D).