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. 2018 Jan 18;8:1103. doi: 10.1038/s41598-018-19429-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MST analysis of PDIs and MPD. (A and B) Dose-response curves of MST analysis (initial fluorescence, n = 3) of PDIA1, PDIA3 and PDIA6 with opMPD (A) or clMPD (B) as indicated in the caption. (C) Summary of the K_D values shown as column chart (error bars indicate respective 68% confidence intervals). The evident differences of the K_D values are not significant (95% confidence intervals overlap, not shown for clarity). (D) The dose-response curves of the initial fluorescence intensity of the control experiment to test for unspecific binding of PDIA1, PDIA3 and PDIA6 to the RED-NHS dye (3 concentrations, each n = 3, curve of PDIA1 against clMPD for reference in grey) show no PDI-concentration-dependent shift of the initial fluorescence.