Table 1.

Ellman’s tests confirmed the successful reduction of analysed PDIs. Upon DTT treatment and buffer exchanges, the percentage of reduced cysteine residues was tested by Ellman’s test. The concentrations of detected free disulfide groups were taken into the relation to the calculated concentration, which was taken from the protein concentration (measured by the absorption at 280 nm) multiplied by the numbers of cysteine residues in the individual PDIs. These concentrations were set to 100% of reduced cysteine residues. PDIA6 was tested in two experimental serials, ones together with PDIA1 and PDIA3, and ones with the indicated TRAP mutants. All tests were performed at least three times independently.

	mean value		mean value		mean value
PDIA1	90.4 ± 11.8	PDIA6	96.5 ± 25.3	PDIA6_C55SC58S	90.5 ± 21.7
PDIA3	77.4 ± 30.4	PDIA6_C58S	115.8 ± 30.4	PDIA6_2 TRAP	102.4 ± 32.2
PDIA6	100.3 ± 4.0	PDIA6_C193S	115.2 ± 15.5	PDIA6_inverse TRAP	126.1 ± 40.3