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. 2018 Jan 18;8:1055. doi: 10.1038/s41598-018-19573-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LPS induces apoptosis in HT29 cells. (A) HT29 cells were exposed to various concentrations of LPS for 24 h. Apoptosis was analysed by Annexin V-FITC/PI double-labelling assay. (B) The degree of apoptotic cell death was quantified. Data represent the mean ± SD (**P < 0.01, ***P < 0.001 compared with control group.). (C) The levels of PLK1 and caspase-3 in HT29 cells after treatment with LPS at various concentrations for 24 h. The graph represents the relative band densities. Values are mean ± SEM (n = 3). **P < 0.01, ***P < 0.001 versus control group.