Table 3.
Binding affinities between PTPδ (Ig1–Fn1) and SALM5 (LRR–Ig)
| PTPδ | SALM5 | K D (μM) | Relative affinity (%) |
|---|---|---|---|
| WT | WT | 14.4 ± 3.2 | 100 |
| PTPδ Ig2–SALM5 LRR | |||
| Q209A | WT | 21.9 ± 0.23 | 66 |
| R233A | WT | 24.8 ± 1.2 | 58 |
| WT | R253A | 95.2 ± 1.7 | 15 |
| L141A | WT | N.D. | — |
| V143A | WT | 150.3 ± 49.5 | 9.6 |
| Y231A | WT | 70.9 ± 7.8 | 20 |
| WT | L249A | 169.1 ± 7.5 | 8.5 |
| PTPδ Ig2–SALM5 Ig | |||
| M137A | WT | 39.9 ± 3.4 | 36 |
| L153A | WT | 25.0 ± 0.27 | 58 |
| WT | I321A | 21.9 ± 2.1 | 66 |
| WT | I358A | 31.4 ± 5.4 | 46 |
| PTPδ Ig3–SALM5 Ig | |||
| Y273A | WT | 77.5 ± 0.63 | 19 |
| M312A | WT | 37.3 ± 1.1 | 39 |
| WT | L288A | 129.4 ± 2.2 | 11 |
WT wild type, N.D. not detectable