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. 2018 Jan 18;8:1114. doi: 10.1038/s41598-017-18915-5

Table 2.

Accuracy of the HRM speciation assay, and of each primer set in the multiplex compared with the 16S rRNA PCR and sequencing.

Target True positive True Negative False Positive False Negative Sensitivity 95% CI Specificity 95% CI
overall 68 43 0 2 97.1% 90.1–99.7% 100% 91.78–100%
A. baumannii 1 112 0 0 100.0% 2.5–100.0% 100.0% 96.8–100.0%
E. coli 37 76 0 0 100.0% 90.5–100.0% 100.0% 95.3–100.0%
Salmonella sp. 1 112 0 0 100.0% 2.5–100.0% 100.0% 96.8–100.0%
K. pneumoniae 25 86 0 2 92.6% 75.7–99.1% 100.0% 95.8–100.0%
K. oxytoca 1 112 0 0 100.0% 2.5–100.0% 100.0% 96.8–99.9%
P. aeuruginosa 3 110 0 0 100.0% 29.2–100.0% 100.0% 96.7–100.0%
16S 99 0 0 14 87.6% 80.1–93.1%