Table 2.
Accuracy of the HRM speciation assay, and of each primer set in the multiplex compared with the 16S rRNA PCR and sequencing.
| Target | True positive | True Negative | False Positive | False Negative | Sensitivity | 95% CI | Specificity | 95% CI |
|---|---|---|---|---|---|---|---|---|
| overall | 68 | 43 | 0 | 2 | 97.1% | 90.1–99.7% | 100% | 91.78–100% |
| A. baumannii | 1 | 112 | 0 | 0 | 100.0% | 2.5–100.0% | 100.0% | 96.8–100.0% |
| E. coli | 37 | 76 | 0 | 0 | 100.0% | 90.5–100.0% | 100.0% | 95.3–100.0% |
| Salmonella sp. | 1 | 112 | 0 | 0 | 100.0% | 2.5–100.0% | 100.0% | 96.8–100.0% |
| K. pneumoniae | 25 | 86 | 0 | 2 | 92.6% | 75.7–99.1% | 100.0% | 95.8–100.0% |
| K. oxytoca | 1 | 112 | 0 | 0 | 100.0% | 2.5–100.0% | 100.0% | 96.8–99.9% |
| P. aeuruginosa | 3 | 110 | 0 | 0 | 100.0% | 29.2–100.0% | 100.0% | 96.7–100.0% |
| 16S | 99 | 0 | 0 | 14 | 87.6% | 80.1–93.1% |