Table 4.
Primer sequences, target genes, amplicon sizes and predicted Tm for the assays.
| Organism | Target gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Concentration (nM) | Amplicon size (bp) | Predicted Tm (°C) | Citation |
|---|---|---|---|---|---|---|---|
| Acinetobacter baumannii | 16S rRNA | CCCACCATGACTTTGACTGG | GGCGCTCTACCAACTAAGCT | 100 | 91 | 76.41 | this study |
| Escherichia coli | uidA | TCTGGCAACCGGGTGAAG | TAGATATCACACTCTGTCTGGCT | 400 | 73 | 77.07 | this study |
| Salmonella sp. | invA | AGCGTACTGGAAAGGGAAAG | CACCGAAATACCGCCAATAAAG | 80 | 123 | 80 | 38 |
| Klebsiella pneumoniae | Khe | CATCTGCCACACCTTTCTCA | CCGGGATTGAGCGGGTAATA | 400 | 105 | 81.12 | this study |
| Gram-negative control | 16S rRNA | AYGACGTCAAGTCMTCATGG | AGGAGGTGATCCAACCGCA | 400 | 353 | 85.65 | 28 |
| Klebsiella oxytoca | pehX | TACCGTCACGCACTATCCTC | TCAAGCGGATACTGGGCC | 400 | 153 | 86.04 | this study |
| Pseudomonas aeruginosa | OatA | CTGGGTCGAAAGGTGGTTGTTATC | GCGGCTGGTGCGGCTGAGTC | 100 | 232 | 89.02 | 39 |