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. 2018 Jan 18;8:1115. doi: 10.1038/s41598-018-19521-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Macrophage secreted factors promote migration of DCs. (a) Expression of the chemokine receptor CCR5 in primary human monocytes (Mo) and monocyte-derived dendritic cells (DCs) was determined by flow cytometry. The average expression of CCR5 in cells from three different donors is depicted. (b) Migration of monocytes (Mo) and DCs in response to conditioned medium (CM) of human monocyte-derived macrophages (HMDM) exposed to 100 µg/mL SWCNT (CM-SWCNT) or 100 µg/mL GO (CM-GO). Cell migration (3 h period) was determined by using transwell chemotaxis microchambers. Data are shown as mean values ± S.D. of three independent experiments; p* < 0.05, Student’s t-test.