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. 2017 Nov 24;41(2):265–279. doi: 10.1007/s00449-017-1864-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Strategy used for the construction of pCMP (a) and pBCS (b). pCMP expression unit for the upstream operon (cmcax and cppAx) with T7 promoter and lac operator, streptomycin resistance region, CloDF13 replicon (CDF ori), lac I region. pBCS expression unit for bcs operon with T7 promoter and lac operator (bcsA, bcsB, bcsC, and bcsC), f1 replicon, ampicillin resistance region, pBR322-derived ColE1 replicon, lac I region