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. 2017 Sep 22;92(1):371–381. doi: 10.1007/s00204-017-2065-z

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Schematic representation of experimental designs for in vitro safety assessment of cosmetic test compounds. a Experimental design to evaluate adverse effects of the cosmetic compounds in hiPSC-CMs under serum-containing culture medium (iCell-MM). b Experimental layout to study adverse effects of the cosmetic compounds in hiPSC-CMs in serum-free (−SR) culture medium; DMEM. Starting day of compound exposure was considered as day 0 in which hiPSC-CMs reached confluency. Following 2-day compound exposure, adverse effects of the compounds were analyzed using different readout assays