Fig. 7.

Overexpression of AHRR does not repress AHRR-regulated CNR2, while overexpression of AHR does not prevent AHR repression of XRCC6 reporter gene activity. COS1 cells were transfected with a CYP1B1–luciferase, b CNR2–luciferase or c XRCC6–luciferase with increasing concentrations of AHR or AHRR and then treated with 10 nM TCDD or DMSO control for 24 h. Integrative genome viewer (IGV) visualization of the AHR- and AHRR-bound peaks in the absence (DMSO) and presence of TCDD for 24 h for b CYP1B1, d CNR2, and f XRCC6. The data are representative of three independent experiments. One-way ANOVA was performed along with Bonferroni post test. Asterisks indicate statistically significant differences (P < 0.05) when compared with the luciferase activity level with 0 ng of AHR, AHRR expression vector transfection. Double asterisks and hashtags indicate statistically significant differences (P < 0.05) compared with the luciferase activity level to the absence of AHRR for DMSO and TCDD treatment, respectively. n.s. not significant