Table 3.
Top 5 overrepresented transcription factor-binding sites (Genomatix, http://www.genomatix.de/) for top 500 TCDD-induced AHR- and AHRR-bound regions
| Rank | Top 500 TCDD-induced AHR-bound regions | Top 500 TCDD-induced AHRR-bound regions | ||||
|---|---|---|---|---|---|---|
| TF families | Description | Z score (genome) | TF families | Description | Z score (genome) | |
| 1 | AHRE | AHR–ARNT heterodimers and AHR-related factors | 34.2 | NRF1 | Nuclear respiratory factor 1 | 94.86 |
| 2 | AP2 | Activator protein 2 | 24.19 | ZF5 | ZF5 POZ domain zinc finger | 88.48 |
| 3 | AP1R | MAF and AP1-related factors | 15.15 | AHRE | AHR–ARNT heterodimers and AHR-related factors | 67.65 |
| 4 | AP1 | AP1, Activating protein 1 | 15.15 | EGR | EGR/nerve growth factor-induced protein C and related factors | 58.42 |
| 5 | ERE | Estrogen response elements | 12.34 | E2F | E2F-myc activator/cell cycle regulator | 50.21 |
The AHRE was ranked first for AHR but third for AHRR. Despite a high z score for AHRE, other GC-rich transcription factor-binding sites were overrepresented in the AHRR dataset with similar or higher significance include nuclear respiratory factor 1 (NRF1), ZF5 POZ domain zinc finger (ZF5), early growth response (EGR), and E2F