Fig. 6.

C2IN-C3lim decreases the recruitment of monocytic cells into the lungs after blunt chest trauma (TXT) of mice in vivo. a Absolute number of monocytes in blood-depleted lungs of mice at 2 and 24 h after sham- or TXT-treatment of the mice. Absolute monocyte number was calculated as the product of whole cell count after lung dissociation/single events measured in flow cytometry * monocytes number in flow cytometry. Monocytes were stained in accordance with Yu et al. (2016) (columns represent mean ± 1 SEM, n = 4 (2 h); n = 6 (24 h), student’s t test; **p ≤ 0.01). b In 7–8 weeks old male C57BL/6 J mice, TXT was induced. Immediately, and 8 h after TXT, phosphate-buffered saline (100 µL PBS) for control or 100 µL C2IN-C3lim (i.e. 1 µg C2IN-C3lim) were instilled intratracheally. 24 h later, bronchoalveolar lavage fluids (BAL) were taken and monocytic cells were counted in a Neubauer chamber after crystal violet staining. Values are given as mean ± SD (n = 8 in each group) and significance was tested using Student’s t test; *p < 0.05 vs. TXT + PBS