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. 2018 Jan 18;8:1118. doi: 10.1038/s41598-017-19063-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representation of Plasmodium vivax circumsporozoite (CS) proteins expressed from Pichia pastoris. (a) Schematic representation of the construction and open reading frame of protein PvCSP-All_FL. (b) PvCSP-All_CT. Sequence repeats in the variant PvCSP-VK210 are indicated in yellow; PvCSP-P. vivax-like in blue; and PvCSP-VK247 in green. (c) Amino acid sequence of the protein yPvCSP-All_FL. (d) Amino acid sequence of the protein yPvCSP-All_CT. Hexa-histidine sequences are indicated in blue.