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. 2018 Jan 18;8:1118. doi: 10.1038/s41598-017-19063-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ratio of IgG1/IgG2c to yPvCSP-All_FL and yPvCSP-All_CT after the immunization schedule. Chimeric proteins yPvCSP-All_FL and yPvCSP-All_CT induced Th2-polarised Abs in C57BL/6 mice. (a) FliC-PvCSP-VK210, (b) FliC-PvCSP-VK247, and c. FliC-PvCSP-P. vivax-like. The results are expressed as the arithmetic mean titres of each group (log₁₀ ± SEM) and were compared by one-way ANOVA followed by Tukey test for multiple comparisons. Significant differences between groups are denoted on the graph as follows: *P < 0.05, **P < 0.01, and ***P < 0.001. Non-significant (ns) differences are also indicated (P > 0.05).