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. 2018 Jan 18;8:1047. doi: 10.1038/s41598-017-18523-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Environmental scanning electron microscopy (ESEM) results. (a) Representative ESEM images of Col-I&III/glycol-chitosan (GCS), Col-III/GCS, positive control (Col-I/GCS) and negative control (CTL/GCS) hydrogels. Porous structures with interconnected pores of irregular shapes were found for the Col/GCS hydrogels. Rounded closed pores with smaller dimensions were found for the negative controls. The pore size increased with the incorporation of collagen in GCS hydrogels; (b) Histograms of the measured pore sizes out of three replicates for each study group. The mode was slightly larger for Col-III/GCS hydrogels, compared with those of associated Col-I&III/GCS hydrogels and positive controls (Col-I/GCS), which were all greater than those of the associated negative controls (Col-III: collagen type III, Col-I: collagen type I, Col-I&III: the simultaneous presence of Col-I and Col-III, and CTL: negative controls. G1 and G2 represent the final GCS concentration of 2% and 1%, respectively).