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. 2018 Jan 18;8:1047. doi: 10.1038/s41598-017-18523-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative fluorescent images of adherent cells cultured on the surface of the collagen (Col)/glycol-chitosan (GCS) hydrogels and negative controls. Actin cytoskeleton and nuclei are shown in red and blue, respectively. Fibroblasts were attached strongly to the surface of the proposed Col/GCS hydrogels. The cells were spread out extensively, and the formation of actin stress fibers were observed. On the contrary, only a few rounded cells were found attached on the surface of the negative controls after 3 days. The images were captured with a 20x objective, and the scale bar shows 100 μm (Col-III: collagen type III, Col-I: collagen type I, Col-I&III: both Col-I and Col-III, and CTL: negative controls. G1 and G2 represent the final GCS concentration of 2% and 1%, respectively).