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. 2018 Jan 18;8:1110. doi: 10.1038/s41598-018-19574-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlations between library concentration estimates and library coverage. A total of 35 Precision ID Ancestry Panel libraries were quantified prior to sequencing using the Qubit (a), TapeStation (b), or ABI7500 qPCR (c) instrument. The ABI7500 was used in combination with the IonLibQuant assay. Linear regression lines (black line) are plotted with 95% confidence interval (grey area). No correlation was observed between concentration estimates and coverage when using Qubit (R² = 7.4*10⁻², p = 0.114) or TapeStation (R² = 6.7*10⁻³, p = 0.651), while the correlation obtained with qPCR was R^2 =0.49 and p = 2.53*10⁻⁶.