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. 2018 Jan 18;8:1086. doi: 10.1038/s41598-018-19373-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence images of Hela and A549 incubated with the FA derived CDs (100 μg/mL) at 37 °C for different incubated time, 0 h (a1,d1), 0.5 h (a2,d2), 1 h (a3,d3), 2 h (a4,d4), and 4 h (a5,d5). As another control, Hela and A549 pre-treated at 37 °C for 2 h with excess FA for FR saturation, and then incubated with CDs (100 μg/mL) at 37 °C for 4 h (a6,d6). Bright field images are shown in b and e. The overlay images are shown in c and f. Scale bar: 10 μm.