Table 1.
TCR repertoire analysis of tumor and spleen in mice under immunotherapy.
| (–) | anti-PD-1 | anti-CTLA-4 | anti-4-1BB | anti-PD-1 + anti-4-1BB | anti-CD4 | ||
|---|---|---|---|---|---|---|---|
| Tumor | No. of total reads/μg RNA (*2) | 6.8 ± 2.8 × 103 | 22.9 ± 11.8 × 103 (p = 0.051) | 22.7 ± 10.5 × 103 (p = 0.051) | 26.6 ± 18.1 × 103 (p = 0.317) | 99.1 ± 105.5 × 103 (p = 0.051) | 46.5 ± 34.0 × 103 (p = 0.051) |
| No. of unique reads (*1) | 2.9 ± 0.8 × 102 | 15.5 ± 7.4 × 102 (p = 0.007) | 16.2 ± 6.3 × 102 (p = 0.004) | 13.3 ± 9.1 × 102 (p = 0.030) | 12.1 ± 2.5 × 102 (p = 0.064) | 12.5 ± 2.1 × 102 (p = 0.050) | |
| Shannon diversity index (*1) | 4.1 ± 0.2 | 5.3 ± 0.9 (p = 0.006) | 5.5 ± 0.4 (p = 0.002) | 5.8 ± 0.5 (p < 0.001) | 4.6 ± 0.6 (p = 0.462) | 4.7 ± 0.4 (p = 0.224) | |
| Spleen | No. of total reads/μg RNA (*1) | 17.3 ± 0.7 × 103 | 37.0 ± 12.5 × 103 (p < 0.001) | 23.5 ± 5.6 × 103 (p = 0.507) | 21.5 ± 6.3 × 103 (p = 0.814) | 20.9 ± 6.3 × 103 (p = 0.890) | 30.1 ± 4.7 × 103 (p = 0.033) |
| No. of unique reads (*1) | 12.9 ± 1.5 × 103 | 8.5 ± 1.4 × 103 (p < 0.001) | 9.6 ± 1.5 × 103 (p = 0.014) | 13.0 ± 2.7 × 103 (p = 1.000) | 8.4 ± 1.2 × 103 (p < 0.001) | 6.3 ± 0.9 × 103 (p < 0.001) | |
| Shannon diversity index (*1) | 9.7 ± 0.2 | 9.1 ± 0.1 (p = 0.042) | 9.3 ± 0.2 (p = 0.372) | 9.1 ± 0.7 (p = 0.058) | 8.2 ± 0.3 (p < 0.001) | 8.8 ± 0.1 (p = 0.002) | |
Dunnett’s test (*1) or Steel’s test (*2) were used for multiple comparisons between control and treatment groups.