Figure 2.
The Rg analysis of heat-activated conformational changes in TRPV1. (a) Shown here is the temperature-dependent change in position-specific Rg (cyan, c30-to-c60; green, C30-to-C60; red, C30-to-C72; blue, SEs of Rg change due to averaging over MD trajectories), in comparison with the Rg change from the closed structure to the open structure of TRPV1 (C-to-O, colored in black). Marker positions (E405, V469, Q498, S510, A566, E651, and N687) for key domains are marked by vertical lines. The residue positions corresponding to the MPD linker, the S1-S2 linker (S12L), the S2-S3 linker (S23L), the S4-S5 linker (S45L), the outer pore, the TRP helix, and the upper/lower gate are marked by horizontal bars colored in green, yellow, cyan, purple, red, blue, and black, respectively. (b) Shown here is a TRPV1 subunit colored by the C30-to-C72 Rg change (with positive/negative change corresponding to red/blue). Outward/inward motions are indicated by arrows colored in red/blue. Marker positions for key domains are shown as spheres and labeled. To see this figure in color, go online.