Figure 6.

VDW interaction energy of lid residue I230 (in hSTING G230I), G230 (in WT hSTING), and I229 (in mSTING) with (a) residues within 5 Å and with (b) only hydrophobic residues (Leu¹⁷⁰, Ile²³⁵, and Tyr²⁴⁰) within 5 Å. The WT hSTING G230 residue has an 8 kcal/mol weaker VDW interaction with its neighboring residues than the Ile found in hSTING G230I and in WT mSTING. However, this difference in VDW interaction energy is not due to the interactions with the neighboring hydrophobic residues as the VDW interaction energies remain similar regardless of whether the residue is a Gly or Ile.