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State-dependent Kir1.1b dimensions measured by LRET between paired Cys on opposite subunits. C189: 33 Å (closed) to 34 Å (open). C289: 27 Å (closed) to 27 Å (open). A161C: 14 Å (closed) to 23 Å (open). Solid bar denotes width of actual HBC gate: 6.3 Å (closed, pH 6) and 15.1 Å (open, pH 8), derived from A161C distances. Open and closed state structures were drawn in Pymol from our homology model (Methods).
