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. 2018 Jan 9;114(1):88–97. doi: 10.1016/j.bpj.2017.10.044

Figure 6.

Figure 6

Normalized LRET lifetimes for C189-Kir1.1b dimer proteoliposomes in 158 mM KMOPS. (A) Closed state at pH 6. (B) Open state at pH 8. C189 dimers were labeled with Tb chelate donor and BODIPY-FL acceptor. Black tracings denote Tb donor-only (DO) signal, fitted with fast and slow Tb time constants (red lines). Green tracings are the corresponding sensitized acceptor (SE) lifetimes at (A) pH 6 (closed state) and (B) pH 8 (open state), together with exponential fits (red lines). Time constants are listed in Table 1, and additional calculations are in Supporting Material. To see this figure in color, go online.