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. 2018 Jan 9;114(1):88–97. doi: 10.1016/j.bpj.2017.10.044

Figure 8.

Figure 8

Normalized LRET lifetimes for A161C-Kir1.1b dimer proteoliposomes in 158 mM KMOPS. (A) Closed state at pH 6. (B) Open state at pH 8. A161C dimers were labeled with Tb chelate donor and Atto465 acceptor. Black tracings denote Tb DO signal, fitted with fast and slow Tb time constants (red lines). Green tracings are the corresponding sensitized acceptor (SE) lifetimes at (A) pH 6 (closed state) and (B) pH 8 (open state), together with exponential fits (red lines). Time constants are listed in Table 3, and additional calculations are in Supporting Material. To see this figure in color, go online.