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Contribution of background proteins to the terbium donor signal. Comparison of Tb DO signals from Cys-free Kir1.1b and A161C-Kir1.1b proteoliposomes. Both samples were produced using identical CF translation and labeling protocols. Red: Tb luminescence from the Cys-free tetrameric sample, representing background (non-Kir) contamination. Blue: Tb luminescence from the A161C tetrameric channel. To see this figure in color, go online.
