Table 3.

LRET Lifetime Decays and Calculated A161C-Kir1.1b Distances between Diagonally Opposite Subunits in the CTD

Data were obtained from energy transfer between Tb donors and Atto-465 acceptors on tetrameric A161C-Kir1.1b, self-assembled in proteoliposomes from single-Cys A161C dimers. τ denotes lifetime decay constants for donors and acceptor sensitized emission, f indicates fast donor state, and s indicates slow donor state. R₀ is the distance corresponding to 50% energy transfer and r is the donor acceptor distance at the designated pH. The average distance between diagonal Cl61 residues in the CTD was 13.8 ± 2 Å at pH 6 and 22.6 ± 6 Å at pH 8.