Table 4.

LRET Distances between Paired Diagonal Cys

Average LRET distances between Cys residues on diagonally opposite subunits. CTD indicates that the Cys pair is in the C-terminal domain. The C161 locus in the A161C mutant is near the HBC gate. The HBC values were calculated from our Kir1.1b homology model, which indicated that C161 was displaced 3.75 Å farther from the central axis of the channel than the actual hydrophobic gate at L160. Therefore, the HBC gate width will be 7.5 Å smaller than the distance between opposite C161 residues.