Table 3.

Optimized Contributions of the CG and AT Basepairs to the Enthalpy, Entropy, and Heat Capacity Increment of Double Helical DNA Dissociation at 25°C

Basepair	ΔCp kJ/K⋅mol-bp	ΔStrans J/K⋅mol	ΔHcoop kJ/mol-bp	ΔSconf J/K⋅mol-bp
CG	0.13 ± 0.01	$R\text{ln}\left(2/\left[No\right]\right)$a	18.8 ± 0.3	44.7 ± 0.2
AT			25 ± 3	72 ± 10

Data from this analysis and from (10) were all obtained at a duplex concentration of 283 μM in 0.15 mM NaCl, pH 7.4.

^aΔS^trans (403 μM) = 70.7 J/K⋅mol; ΔS^trans (337 μM) = 72.2 J/K⋅mol; ΔS^trans (283 μM) = 73.7 J/K⋅mol; ΔS^trans (214 μM) = 76.0 J/K⋅mol; ΔS^trans (107 μM) = 81.7 J/K⋅mol; ΔS^trans (87 μM) = 83.4 J/K⋅mol; and ΔS^trans (40 μM) = 90.0 J/K⋅mol.