Table 4.

The Melting Temperatures of Various DNA Duplexes Calculated Using the Data Given in Table 3 for CG and AT Pairs

DNA Duplexes	Composition	References	Concentration μM	Tt °C Experiment	Tt °C Calculated
5′-CGCCGCCGC-3′	9-CG	(10)	283	74.0	73.5
3′-GCGGCGGCG-5′
5′-CGCCGCCGCCGC-3′	12-CG	(10)	283	83.6	83.1
3′-GCGGCGGCGGCG-5′
5′-CGCCGCCGCCGCCGC-3′	15-CG	(10)	283	89.5	89.3
3′-GCGGCGGCGGCGGCG-5′
5′-CGCCGCCGCCGCCGCCGCCG-3′	20-CG	N/Aa	283	N/A	94.2
3′-GCGGCGGCGGCGGCGGCGGC-5′
5′-CGCCGCCGCCGCCGCCGCCGCGCGC-3′	25-CG	N/Aa	283	N/A	98.7
3′-GCGGCGGCGGCGGCGGCGGCGCGCG-5′
5′-CGCAAACGC-3′	6CG, 3AT	(10)	283	60.4	60.3
3′-GCGTTTGCG-5′
5′-CGCAAATTTCGC-3′	6CG, 6AT	(10)	283	64.5	62.1
3′-GCGTTTAAAGCG-5′
5′-CGCAAAAAACGC-3′	6CG, 6AT	(10)	283	63.0	62.1
3′-GCGTTTTTTGCG-5′
5′-CGCTTTAAACGC-3′	6CG, 6AT	(10)	283	60.8	62.1
3′-GCGAAATTTGCG-5′
5′-CGCATATATCGC-3′	6CG, 6AT	(10)	283	60.3	62.1
3′-GCGTATATAGCG-5′
5′-CGCAAATTTAAACGC-3′	6CG, 9AT	(10)	283	64.8	63.3
3′-GCGTTTAAATTTGCG-5′
5′-CGCAAAAAAAAACGC-3′	6CG, 9AT	(10)	283	65.1	63.3
3′-GCGTTTTTTTTTGCG-5′
5′-CGCAGAGAGAGACGC-3′	10CG, 5AT	N/Ab	283	72.2	73.6
3′-GCGTCTCTCTCTGCG-5′
5′-CGCACACACACACGC-3′	10CG, 5AT	N/Ab	283	75.9	73.6
3′-GCGTGTGTGTGTGCG-5′
5′-CGAACAATCG-3′	5CG, 5AT	(9)	214	51.3	57.1
3′-GCTTGTTAGC-5′
5′-CGAACAATCG-3′	5CG, 5AT	(9)	107	49.0	53.8
3′-GCTTGTTAGC-5′
5′-GCGAACAATCGG-3′	7CG, 5AT	(9)	403	64.8	67.3
3′-CGCTTGTTAGCC-5′
5′-GCGAACAATCGG-3′	7CG, 5AT	(9)	87	60.6	61.5
3′-CGCTTGTTAGCC-5′

Experimental data from (9, 10) are given where available.

^aCalculated T_t values only.

^bPreviously unpublished experimental data from our laboratory.