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Analysis of domain-specific TRITC-DHPE diffusivity (A) and Eraft data of NBD-DHPE distribution (B) suggest that variation in membrane CHOL between 25 and 37 mol% CHOL alters lipid packing differences between lo and ld domains of a polymer-tethered lipid bilayer containing DOPC/DPPC/CHOL mixtures (1:1 DOPC/DPPC molar ratio); p < 0.005. Error bars represent standard deviation of data sets from 15 different bilayer regions (out of three samples).
