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. 2018 Jan 9;114(1):158–167. doi: 10.1016/j.bpj.2017.11.005

Figure 4.

Figure 4

Representative time scans of confocal fluorescence intensity (A) and corresponding PCH curves (B) of Alexa 555 MAb-labeled αvβ3 in lo and ld domains in a polymer-tethered lipid bilayer comprised of an equimolar ratio of DOPC and DPPC and 33 mol% CHOL. Parameter Xdimer from PCH analysis demonstrates that changes in CHOL content do not alter the predominantly monomeric nature of integrins in the bilayer (C) (p < 0.01). Data in (B) and (C) are based on 50 independent data sets for each lipid composition. Errors bars represent standard deviation.