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Scheme for labeled protein production. Top: Direct incorporation of p-cyanophenylalanine (Cnf) and tryptophan (Trp) via unnatural amino acid mutagenesis and conventional mutagenesis, respectively. Bottom: Incorporation of Cysteine (Cys) and O-propargyl tyrosine (Ppy) with subsequent labeling by fluorescein-5-maleimide (Fam) and tetramethylrhodamine azide (Raz), respectively. To see this figure in color, go online.
