Figure 9.

Charge of chymosin versus salt concentration. The red horizontal line shows the original monopole charge, $n_0$, reported in Table 1. The green line shows the number of anions bound to the protein, $n_{\text{b}}$, whereas the blue line shows the net protein charge, $n_0-n_{\text{b}}$, after anion binding. (A) At pH 4.0, anion binding results in a reversal in the protein charge. (B) At pH 4.6, anion binding enhances the monopole charge as the salt concentration increases. $N_{\text{s}}=36,E_{\text{b}}=-0.3k_{\text{B}}T$. To see this figure in color, go online.