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Primer Sequences for Methylated and Unmethylated DNA Template
| Gene | Primer sequence | Annealing temperature (°C) | Amplicon size |
|---|---|---|---|
| ATM | MF 5’-GGAGTTCGAGTCGAAGGGC-3’ | 59 | 239bp |
| MR 5’-CTACCTACTCCCGCTTCCGA-3’ | |||
| UF 5’GTTTTGGAGTTTGAGTTGAAGGGT-3’ | 56 | 246bp | |
| UR 5’-AACTACCTACTCCCACTTCCAA-3’ |
