Table 1.
Domain/ | Primer | Sequence | Amplicon size (bp) |
---|---|---|---|
Subdomain | |||
DI | DI-Psec-S: | 5′-GGTTTTTCTTAAGCTTGACCCAAGTGTGCACCG-3′ | |
DI-Psec-AS: | 5′-AAGAAAAAAACTCGAGCGCGGTTGGTGTC-3′ | 512 | |
DII | DII- Psec -S: | 5′-GGTTTTTCTTAAGCTTGCCCCAGCTCTGCTAC-3′ | |
DII- Psec -AS: | 5′-AAGAAAAACCCTCGAGCCTTGCTGCACTTCTC-3′ | 548 | |
DIII | DIII- Psec -S: | 5′-GGTTTTTCTTAAGCTTGGACGTGGGATCCTGC-3′ | |
DIII- Psec –AS: | 5′-AAGAAAAACCCTCGAGCCACACACTCGTCC-3′ | 611 | |
DIV | DIV- Psec -S: | 5′-GGTTTTTCTTAAGCTTGTTCGTGCACACGGTGC-3′ | |
DIV- Psec -AS: | 5′-AAGAAAAAAAACTCGAGCCGTCAGAGGGC-3′ | 545 | |
Full ECD | Full ECD-Psec-S | 5′-GGTTTTTCTTAAGCTTGACCCAAGTGTGCACCG-3′ | |
Full ECD-Psec-As | 5′-AAGAAAAAAAACTCGAGCCGTCAGAGGGC-3′ | 2014 |
S, sense;AS, anti-sense;underlined sequences denote restriction enzyme sites