Table 1.
Specific Primers Used for Subcloning of Extracellular Domain and Subdomains of HER2
| Domain/ | Primer | Sequence | Amplicon size (bp) |
|---|---|---|---|
| Subdomain | |||
| DI | DI-Psec-S: | 5′-GGTTTTTCTTAAGCTTGACCCAAGTGTGCACCG-3′ | |
| DI-Psec-AS: | 5′-AAGAAAAAAACTCGAGCGCGGTTGGTGTC-3′ | 512 | |
| DII | DII- Psec -S: | 5′-GGTTTTTCTTAAGCTTGCCCCAGCTCTGCTAC-3′ | |
| DII- Psec -AS: | 5′-AAGAAAAACCCTCGAGCCTTGCTGCACTTCTC-3′ | 548 | |
| DIII | DIII- Psec -S: | 5′-GGTTTTTCTTAAGCTTGGACGTGGGATCCTGC-3′ | |
| DIII- Psec –AS: | 5′-AAGAAAAACCCTCGAGCCACACACTCGTCC-3′ | 611 | |
| DIV | DIV- Psec -S: | 5′-GGTTTTTCTTAAGCTTGTTCGTGCACACGGTGC-3′ | |
| DIV- Psec -AS: | 5′-AAGAAAAAAAACTCGAGCCGTCAGAGGGC-3′ | 545 | |
| Full ECD | Full ECD-Psec-S | 5′-GGTTTTTCTTAAGCTTGACCCAAGTGTGCACCG-3′ | |
| Full ECD-Psec-As | 5′-AAGAAAAAAAACTCGAGCCGTCAGAGGGC-3′ | 2014 |
S, sense;AS, anti-sense;underlined sequences denote restriction enzyme sites