Table 3.
Binding Profiles of mAbs to Recombinant Subdomains of HER2-ECD by ELISA
| mAbs | DI | DI+DII | DII | DIII | DIII+DIV | DIV | HER2-ECD |
|---|---|---|---|---|---|---|---|
| 1F2 | ++ | ++ | - | - | - | - | ++ |
| 1T0 | - | ++ | - | - | - | - | ++ |
| 2A8 | - | - | - | - | ++ | - | ++ |
| 2A9 | ++ | ++ | - | - | - | - | ++ |
| 1H6 | ++ | ++ | - | - | - | - | ++ |
| 1H9 | - | - | - | - | ++ | ++ | ++ |
| 1B5 | - | - | - | - | ++ | - | ++ |
| 4C7 | - | - | - | + | ++ | - | ++ |
| Pertuzumab | - | - | - | - | - | - | ++ |
| Trastuzumab | - | - | - | - | ++ | - | ++ |
| Anti-HER2 polyclonal Ab | ++ | ++ | - | + | ++ | ++ | ++ |
| Anti –His Ab | + | ++ | ++ | + | ++ | ++ | ++ |
Trastuzumab, Pertuzumab and anti-HER2 polyclonal antibodies are used as positive controls. Full HER2-ECD is also employed as positive control in the coating layer. Anti-His mAb was used to verify the coated recombinant proteins. The results represent OD values of <0.2 (-), 1.0-2.0 (+) and >2.0 (++)