Fig. 2.

Effects of dietary Pi on renal α-Klotho gene expression in each age group mice. 1, 2 and 13 month-old C57BL/6J mice were fed either a HP (□) or LP (■) diet for 5 days. Total RNA from the kidneys was extracted and gene expression was measured by quantitative real-time RT-PCR analysis. (A) Renal α-Klotho mRNA expression. Data are expressed as means ± SEM (n = 4–5). ^†p<0.05 vs 1 month-old group, ^††p<0.05 vs 2 month-old group. Whole protein samples from mouse kidney tissues (20 µg) were analyzed by western blotting. (B) α-Klotho protein expression.