Table 2.
The primer sequences for PCR amplification
| Gene name | Forward sequence (5' to 3') | Reverse sequence (5' to 3') | Gene accession NM |
|---|---|---|---|
| Cyp27b1 | ATGGTGAAGAATGGCAGAGG | TAGTCGTCGCACAAGGTCAC | NM_010009 |
| Cyp24a1 | TCAAGCCAGCGTTCGGGTCTAA | TGCCATTCACAACTCGGACCCT | NM_009996 |
| α-Klotho | CAAAAGCTGATAGAGGACAATGGC | GGCAGAGAAATCAACACAGTAAGG | NM_013823 |
| Fgfr1 | CCAGTCATCCATGAACTCTGGGGTTCTCC | GGTCACACGGTTGGGTTTGTCCTTATCCAG | NM_010206 |
| Fgf23 | ATGCTAGGGACCTGCCTTAGA | AGCCAAGCAATGGGGAAGTG | NM_022657 |
| β-actin | CTGACCCTGAAGTACCCCATTGAACA | CTGGGGTGTTGAAGGTCTCAAACATG | NM_007393 |