Table 3.
Photolytic production methods of multiple reactive oxygen species
| Free radical | Spin trap | Precursor/Sensitizer | UV/VL | Irradiation period | Antioxiidant equivalent |
|---|---|---|---|---|---|
| •OH | CYPMPO | H2O2 10 mM | UV | 5 s | GSH |
| O2•− | CYPMPO | Riboflavin 20 µM | VL | 60 s | SOD |
| RO• | CYPMPO | AAPH 10 mM | UV | 5 s | Trolox |
| ROO• | CYPMPO | tert-BHP 10 mM | UV | 5 s | α-lipoic acid |
| 1O2 | TMPO | Rosebengal 200 µM | VL | 30 s | GSH |
UV, ultraviolet (300–400 nm); VL, visual light (500–600 nm); CYPMPO, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide; AAPH, 2,2'-azobis (2-amidinopropane) hydrochloride; tert-BHP, tert-butyl hydroperoxide; GSH, glutathione; SOD, superoxide dismutase; TMPO, 4-hydroxy-2,2,6,6-tetramethylpiperidine.