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. 2018 Jan 17;5(2):e437. doi: 10.1212/NXI.0000000000000437

Figure 5. Mononuclear cell subset isolation from spinal cord of mice with experimental autoimmune encephalomeylitis.

Figure 5

There was no significant difference between the 2 experimental groups regarding (A) CD3+ T cells, (B) CD4+ T cells, (C) CD8+ T cells, (D) CD19+ B cells, and (E) CD11b+ myeloid cells. Finally, the fourth requirement of our assay qualification algorithm, namely a correlation between absolute mononuclear cell numbers in brain and in spinal cord and experimental autoimmune encephalomyelitis (EAE) disease course was tested. (F) There was a significant correlation between live mononuclear cell numbers isolated from spinal cord tissue with Kit and clinical EAE disease severity. Analyses of mononuclear cell subsets in the brain or spinal cord did not demonstrate any significant correlations with EAE disease activity, regardless of the tissue dissociation method that was utilized. Each experiment was repeated at least once. Representative results are shown. Groups were compared for normality by using the Kolmogorov-Smirnov test. Values were compared using the non-parametric test Mann-Whitney U test.